يمكنكم ارسال مقالاتكم عبر البريد الالكتروني jscience@ush.sd

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Identification of Methylation Status of IGF2/ H19 genes and Cell Blood Count among Neonates with Low Birth Weight

Emad A. Nourelgalil,Ali Mahmoud Mohammed Edris, Abdalla M. Abdalla & Fathelrahman M. Hassan

1Emad A. Nourelgalil, 2,Ali Mahmoud Mohammed Edris, 4Abdalla M. Abdalla & 5Fathelrahman M. Hassan

1Department of Hematology & Immunohematology, Faculty of Medical Laboratory Science, Managil University of Science & Technology, Managil, Sudan.

2Faculty of Medical Laboratory Science, University of Khartoum, Sudan

3Department of Hematology & Immunohematology, Faculty of Medical Laboratory Science, Sudan University of Science & Technology, Khartoum, Sudan

4Department of Hematology & Immunohematology, College of Medical Laboratory Science, Sudan University of Science and Technology, Khartoum, Sudan

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Abstract

 

Introduction: Notably, Insulin-like growth factor 2 and H19 genes are two of the imprinted candidate genes that are involved in fetal growth and development. Accordingly, alteration in DNA methylation of imprinted genes and haematological parameters has been repeatedly associated with low birth weight through intrauterine growth retardation. The mechanisms underlying this association are unknown, although epigenetic mechanisms are hypothesized; so, this was conducted to evaluate the association between alteration in DNA methylation of imprinted genes and haematological parameters and birth weight. Methods: This is a prospective cross sectional study conducted in White Nile State (Sudan). The birth weights (BW) of the newborns were recorded soon after delivery. Then 29 cord blood samples were randomly selected from neonates with low birth weight as cases and 44 cord blood samples from neonates with normal birth weight as controls and subsequently tested for IGF2/H19 methylation status in imprinting control region (ICR) and haematological parameters via Methylation Specific PCR (MSP) and Auto hematological Analyzer (Mindary 3000) respectively. Results: Our results showed no differences between cases and control in imprinting control region (ICR), that, the IGF2 and H19 imprinting genes were seen in unmethylation status in imprinting control region (ICR) of two groups. Although, there are no significance differences in haematological parameters between cases and control group in relation to birth weight. Conclusion:Our findings revealed that the DNA methylation of imprinted genes and haematological parameters are not influence by reduction in neonatal birth weight. The IGF2 and H19 imprinting genes were seen in unmethylated status, indicating that the two genes remain actively in the imprinting control area and are unaffected by reduction in birth weight.
Keywords: Cord, epigenetics, imprinting, placenta, methylation.

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