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Comparative Study of Chemical Constituents of the Seeds Extract of Acacia Seyal and Acacia nilotica and its effect as antimicrobial and antioxidant

Hassan. E. Elkhidr; Asrar Adil Algray

Hassan. E. Elkhidr1, Asrar Adil Algray2*

1 Department of chemistry, Faculty of Science and Technology, Shendi University, Shendi, Sudan. elkhdirhassan@gmail.com

2 Department of chemistry, Faculty of Science and Technology, Shendi University, Shendi, Sudan, asraradil123@ush.sd

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Abstract

This study was carried out to investigate the phytochemical constituents of the seeds extract of Acacia seyal, and Acacia nilotica and to study the constituents of their oils and to test the antimicrobial and antioxidant effect of the constutionts. Phytochemical screening revealed the presence of flavonoids, alkaloids, saponins, tannins, carbohydrates, phenols, glycosides, cardiac glycosides, quinones, anthraquinone, terpenoids, coumarins and steroids in all extracts. Quantitative analysis of some important bioactive constituents was carried out. The seeds oil were analyzed by GC-MS. The GC-MS revealed that the dominant constituents in Acacia seyal are: 9,12-octadecadienoic acid (Z,Z)-, methyl ester (30.19%), methyl stearate (15.77%), 9-octadecenoic acid (Z)-, methyl ester (15.57%), eicosanoic acid, methyl ester (6.89%), docosanoic acid, methyl ester (6.67%), 11,14-eicosadienoic acid, methyl ester (4.16%), tetracosanoic acid, methyl ester (3.73%), cis-11-Eicosenoic acid, methyl ester (3.17). The most dominant constituents in the seeds oil of Acacia nilotica are: 9,12-octadecadienoic acid (Z,Z), methyl ester (48.77%), 9-octadecenoic acid (Z)-, methyl ester (28.50%), hexadecanoic acid, methyl ester (9.44%), methyl stearate (8.75%). The oils were evaluated for their antimicrobial potential against standard pathogenic microbes. In the disc diffustion bioassay, Acacia seyal oil showed good activity against Bacillus subtilis, Staphylococcus aureu and Pseudomonas aeruginosa and good anticandidal potency. However, it showed weak activity against Escherichia coli. The oil of Acacia nilotca showed good activity against Bacillus subtilis, Staphylococcus aureus and Escherichia coli with good anticandidal potency. It also showed weak activity against Pseudomonas aeruginosa. The antioxidant activities of the oils were evaluated using 1,1-diphenyl-2-picrylhydraxyl (DPPH). The oils of Acacia seyal and Acacia nilotica showed very high DPPH radical scavenging activity. It seems that the oil of two spices is a lead for further optimization.

Keywords: Acacia Seyal, Acacia Nilotica, Fixed oil, GC-MS, Antimicrobial Activity.

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