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Detection of aminoglycosides resistance among strains of Klebsiella pneumoniae and Pseudomonas aeruginosa isolated from patients with urinary tract infection

Hadia Mohammed Idrees, Waseem Sameer Kwami*, Tanzeel Elsadig Ibrahim, Ansam Ali Ahmed

Department of Microbiology, Faculty of Medical Laboratory Sciences, Shendi University, Shendi, Sudan, *sameerw77@yahoo.com

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Abstract

This study aims to detect the resistance pattern of aminoglycosides among isolated strains of Klebsiella pneumoniae and Pseudomonas aeruginosa from patients with urinary tract infection (UTI). A total of 70 urine samples were collected from patients with symptoms of urinary tract infection in sterile urine containers, and then inoculated in CLED media, incubated at 37 °C for 24 hours. Species of K. pneumoniae and P. aeruginosa were identified according to their colonial morphology, indirect gram staining reaction and biochemical tests. Identified species were tested for antimicrobial susceptibility against the following aminoglycosides: Amikacin, Kanamycin, Streptomycin, Gentamicin, and Tobramycin using the Kirby-bauer disc diffusion method. Results revealed that the overall resistance of K pneumoniae to aminoglycosides was 16% and 33.3% for P. aeruginosa. In conclusion, the most powerful aminoglycoside against K. pneumoniae isolated from UTI patients was Gentamicin, Amikacin and Kanamycin, and the most powerful antibiotic against Pseudomonas species was Amikacin and Gentamicin respectively.
Keywords: Aminoglycosides, resistance, Klebsiella pneumoniae, Pseudomonas aeruginosa, UTI.

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