Ibrahim Bakhit Yousif Elemam1*, Sara Ali Abdalla Ali1, , Shyma Alzubair2, Monier Hassan Ibrahim1, Husam Elden AlNour1, Osman Mohammed El mahi Hassan3.
1* Department of Histopathology & Cytology, Faculty of Medical Laboratory Sciences, Shendi University, Shendi, Sudan. elemam.ibrahim@yahoo.com
1 M.Sc Department of Histopathology & Cytology, College of Medical Laboratory Science, Al-Neelain University, Khartoum, Sudan.
3 M.Sc Department of Histopathology & Cytology, College of Medical Laboratory Science, Sudan University of Science and technology, Khartoum, Sudan.
4 Assosiate Profof Histopathology and cytology in Karary University, Khartoum, Sudan.
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Abstract
Prostate cancer is the second most common and fifth most aggressive neoplasm among men worldwide. PC is a manifested heterogeneous disease, ranging from slow growing and indolent to aggressive with high risk of fatal outcome. The study investigated the detection of P53 expression by using immunohistochemical method and Ag NOR score in prostate cancer and to correlate between them among Sudanese patients. Forty six paraffin block samples were collected from samples previously diagnosed as prostate cancer from Military hospital in Khartoum state within 2019-2020, from the block two sections was cut at 3µm thickness by rotary microtome. One for P53 immunohistochemical staining method and the other for Ag NOR score sliver staining method. Patient’s age ranged between 55 and 89 years with mean age of 72±7.654 years, most prostate cancer patients were at sixth and seventh decades. The positive expression of P53 were seen in 15/46 (32.61%) samples while negative expiration in 31/46 (67.39%) samples. The P53 expression was (0%, 2.17%, 6.52%, 6.52%, 15.22% and 4.35%) in Gleason score (5, 6, 7, 8, 9 and 10) respectively, there was insignificant statistical correlation between P53 expression and Gleason score (P-value 0.051). Ag NORs score was (2.66) among P53 positive samples and (2.20) in P53 a negative samples, there was a significant statistical correlation between Ag NORs score and P53 expression (P-value 0.011). Ag NORs score was (1.88, 2.33, 2.24, 2.36, 2.75, and 2.14) in Gleason score (5, 6, 7, 8, 9 and 10) respectively, this result shows insignificant statistical correlation between Ag NORs score and Gleason score (P-value 0.355).
Keywords: Prostate cancer, P53, Ag NORs.