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Molecular Detection of Human Papilloma Virus Type 16 and 18 among Sudanese Patients with Prostate Mass

Ibrahim Bakhit Yousif Elemam, Ahmed Ibn Edriss Mohamed, Ayat Salah M. Suliman

Ibrahim Bakhit Yousif Elemam1, Ahmed Ibn Edriss Mohamed2, Ayat Salah M. Suliman3

1 Department of Histopathology & Cytology, Faculty of Medical Laboratory Sciences, Shendi University, Shendi, Sudan.
2 Department of Histopathology & Cytology, Faculty of Medical Laboratory Sciences, University of El Imam EL Mahdi, Kosti, Sudan.
3 BSc. Medical Laboratory Science, Shendi University.


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Abstract

Background and Objective: Despite the rising number of research recently conducted to assess the association between Human Papillomavirus (HPV) infections and prostate cancer, the results remain disputed; therefore, we try to investigate the possible role of HPV 16 and 18 as a risk factor for prostate cancer and benign prostatic hyperplasia in Sudanese patients. Method: This is a cross-sectional retrospective study conducted on formalin fixed paraffin wax embedded (FFPE) tissues, retrieved from the archives of histopathology Laboratory at Omdurman teaching hospital (Sudan). 200 samples of FFPE prostate tissue blocks from 2020 to 2022 were selected for this study. A 100 were prostate adenocarcinoma and 100 were benign prostatic hyperplasia. All samples were of Sudanese ethnic, sections of 10 μm prepared by sterile microtome blade, and then deparaffinized and the DNA extracted chemically and stored at – 20 °C. HPV 16 and 18 was investigated on all samples using PCR. The data analyzed by Statistical Package for the Social Sciences (SPSS) software version 19. Results: The results showed that HPV-16 was positive in 6(6%) of the adenocarcinoma and 4(4%) of the hyperplasia. The difference of HPV-16 infection between prostate adenocarcinoma and benign prostatic hyperplasia was statistically in significant (P. Value 0.374). HPV-18 was positive in 2(2%) of the adenocarcinoma and 4(4%) of the hyperplasia also with no statistical significant difference (P. Value 0.341), also there was in significant statistical correlation between HPV type 16 and 18 with prostate cancer grade as the p. value was (0.60 and 0.925) respectively. Conclusion: These findings excluded the role of HPV 16 and 18 in prostate cancer in Sudanese.
Keywords: Molecular detection; HPV 16 and 18 ; prostate masses ; Sudanese men

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