يمكنكم ارسال مقالاتكم عبر البريد الالكتروني jscience@ush.sd

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Phytochemical Screening and Investigation of Oil of Acacia tortilis seeds

Asrar Adil Algray, Hassan Elamein Elkhidr

Asrar Adil Algray1, Hassan Elamein Elkhidr2*

1)Department of chemistry, Faculty of Science and Technology, Shendi University, Shendi, Sudan, *asraradil123@ush.sd

2)Department of chemistry, Faculty of Science and Technology, Shendi University, Shendi, Sudan.

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Abstract

This study was carried out to assess the quality and quantity of some phytochemicals present in the seeds of Acacia tortilis as well as to study the constituents of its oil and to evaluate the antimicrobial and antioxidant effect of it. Phytochemical screening revealed the presence of Flavonoids, Alkaloids, Saponins, Tannins, Carbohydrates, Phenols, Glycosides, Cardiac Glycosides, Quinones, Anthraquinone, Terpenoids, Coumarins and Steroids are present in Acacia tortilis extract. Quantitative analysis of some important bioactive constituents was carried out. The oil was analyzed by GC-MS. GC-MS revealed that the dominant constituents in Acacia tortilis oil are; 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (46.50%), 9-Octadecenoic acid (Z)-, methyl ester (23.48%), Hexadecanoic acid, methyl ester (12.64%), Methyl stearate (8.40%), Docosanoic acid, methyl ester (2.65%), Eicosanoic acid, methyl ester (2.61%). The oil was evaluated for its antimicrobial potential against standard pathogenic microbes. In the disc diffusion bioassay, Acacia tortilis oil showed good activity against Bacillus subtilis. Escherichia coli and Staphylococcus aureus. It also showed weak anticandidal potency. The oil had no activity against Pseudomonas aeruginosa. The antioxidant activities of the oil were evaluated using 1,1-diphenyl-2-picrylhydraxyl (DPPH). The oil of Acacia tortilis showed very high DPPH radical scavenging activity.

Keywords: Acacia tortilis, antioxidant, antimicrobial, Phytochemical

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